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Brilliant Violet 650(TM) anti-mouse CD25

Brilliant Violet 650(TM) anti-mouse CD25

Cat no: 102037

Supplier: BioLegend
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CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Ralpha, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rbeta) and CD132 (common gamma chain), CD25 forms the high affinity signaling IL-2 receptor.
Catalogue number: 102037
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry
Size: 125 microl
Clone: PC61
Gene: 16184
Dilutions: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ?5 microl per million cells or 5 microl per 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 650(TM) excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650(TM) is a trademark of Sirigen Group Ltd.
Storage buffer: The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.
Antigen: IL-2-dependent cytolytic mouse T-cell clone B6.1
Isotype: Rat IgG1, lambda
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: IL-2Ralpha, Ly-43, p55, Tac
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