Brilliant Violet 650(TM) anti-human CD123

CD123 is the 70 kD transmembrane alpha chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CDw131 (common beta chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the beta chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.

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SPECIFICATIONS

Catalog Number

306019

Size

25 tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Clone

6H6

Antigen

Human IL-3Ralpha transfected COS cells.

Gene Id

3563

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ?5 microl per million cells or 5 microl per 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 650(TM) excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650(TM) is a trademark of Sirigen Group Ltd.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.

Alternative Names

IL-3Ralpha, IL-3 Receptor alpha

Regulatory Status

RUO