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Brilliant Violet 605(TM) anti-human CD25

Brilliant Violet 605(TM) anti-human CD25

Cat no: 302632

Supplier: BioLegend
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CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor alpha chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor beta (CD122) and common gamma chains (CD132) to form the high affinity IL-2R complex.
Catalogue number: 302632
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100 tests
Clone: BC96
Gene: 3559
Dilutions: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ?5 microl per million cells or 5 microl per 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 605(TM) excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605(TM) is a trademark of Sirigen Group Ltd.
Storage buffer: The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.
Isotype: Mouse IgG1, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Low affinity IL-2R, IL-2R alpha chain, Tac, p55
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