Brefeldin A Solution (1,000X)

Brefeldin A (BFA) is a protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation. Especially useful for the intracellular staining of cytokines, BFA leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum (see Jung, et al., 1993). Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest (see references below for additional information). Brefeldin A Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.

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SPECIFICATIONS

Catalog Number

420601

Size

1 ml

Applications

FC, ICC

Dilutions

Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with brefeldin A for ? 24 hours, as this can become toxic for cell viability.

Storage Temp

4 C

Concentration

5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

Store the Brefeldin A Solution (1,000X) at 4C. Note: DMSO freezes at 4C.

Alternative Names

BFA

Regulatory Status

RUO