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Biotin anti-mouse IL-1alpha

Biotin anti-mouse IL-1alpha

Cat no: 512503

Supplier: BioLegend
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IL-1 refers to two proteins, IL-1alpha and IL-1beta which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1alpha is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses. The biotinylated BL1a-89 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay when used in conjunction with the purified BL1a-72 antibody as capture antibody and recombinant mouse IL-1alpha?(Cat. No. 562801) as the standard.
Catalogue number: 512503
Reactivities: Mouse
Hosts: Rat
Applications: Immunoprecipitation, Western Blot
Conjugates: Biotin
Size: 50 microg
Clone: BL1a-89
Gene: 16175
Dilutions: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.05-0.2 microg/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-1alpha recobinant protein ranging from 250 to 4 pg/ml are recommended for each ELISA plate. For Western Blotting applications, it is suggested to use 5 microg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: The antibody solution should be stored undiluted at 4C. Do not freeze.
Concentration: 0.5 mg/ml
Antigen: E. coli - expressed, recombinant mouse IL-1alpha
Isotype: Rat IgG2a, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Interleukin-1alpha, IL-1F1, LAF, EP, LEM, MCF/MNCF
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