Biotin anti-human MCP-1

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 5D3-F7 antibody reacts with human monocyte chemoattractant protein-1 (MCP-1).

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SPECIFICATIONS

Catalog Number

502608

Size

50 microg

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum

Conjugates

Biotin

Clone

5D3-F7

Antigen

Recombinant human MCP-1

Gene Id

6347

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2 microg/ml is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. For immunofluorescent staining, the suggested use of this reagent is ? 0.25 microg per 106 cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C. Do not freeze.

Alternative Names

Monocyte chemoattractant protein-1, MCAF, JE, SCYA2, HC-11, P6, SMC-CF, CCL2

Regulatory Status

RUO