Biotin anti-human CD140a (PDGFRalpha)

The 16A1 monoclonal antibody recognizes human CD140a also known as the platelet-derived growth factor receptor, alpha polypeptide, PDGFR2, and PDGFRalpha. CD140a is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The identity of the growth factor bound to the receptor determines whether the functional receptor is a homodimer or heterodimer composed of both PDGFR-alpha and -beta. CD140a contains three immunoglobulin-like domains and a tyrosine kinase domain with a predicted molecular weight approximately 123 kD. CD140a is widely expressed on a variety of mesenchymal-derived cells and has been implicated in the development of some tumors including basal cell carcinoma and gastric stromal cell tumors. Binding of A-chain containing PDGF molecules as well as protease-activated PDGF-C molecules can stimulate cell proliferation. CD140a has been shown to interact with a number of proteins including CRK, Grb2, Grb14, SHP2, and others as integrin beta3, caveolin-1, and nexin sorting molecules. The PDGFRalpha is heavily phosphorylated on numerous tyrosine residues through both autophosphorylation and ligand-dependent processes. The 16A1 antibody has been shown to be useful for flow cytometric detection of CD140a.

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SPECIFICATIONS

Catalog Number

323504

Size

100 microg

Applications

FC, IF

Hosts

Mouse

Reactivities

Hum

Conjugates

Biotin

Clone

16A1

Antigen

NIH 3T3 cells transfected with human PDGFRalpha

Gene Id

5156

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ?2.0 microg per million cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C. Do not freeze.

Alternative Names

PDGFR2, PDGFRalpha

Regulatory Status

RUO