Biotin anti-human C5a/C5a(desArg)/C5

C5, complement component 5, is a protein involved in the complement system. It is cleaved into C5a and C5b by the C3/C5 convertase. The smaller fragment C5a is an anaphylatoxin and is released into the fluid surroundings. C5a is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis). C5a contains 74 amino acids and is rapidly metabolised by a serum enzyme, carboxypeptidase B to a 73 amino acid form, C5a des-Arg. The major fragment C5b binds to the membrane initiating the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex.

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SPECIFICATIONS

Catalog Number

518306

Size

500 microg

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum

Conjugates

Biotin

Clone

G25/2

Antigen

Human C5a

Gene Id

727

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25-1 microg/ml is recommended. To obtain a linear standard curve, serial dilutions of C5a recombinant protein ranging from 15.6 to 1000 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.

Alternative Names

C5a, C5a desarg, C5, complement component 5, complement C5a

Regulatory Status

RUO