APC anti-mouse F4/80

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8 T cells-mediated peripheral tolerance.

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SPECIFICATIONS

Catalog Number

123116

Size

100 microg

Applications

FC

Hosts

Rat

Reactivities

Mouse

Clone

BM8

Antigen

Murine macrophages

Gene Id

13733

Isotype

Rat IgG2a, kappa

Clonality

Monoclonal

Dilutions

Each lot of this F4/80 antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ? 0.25 microg per 106 cells in 100 microl. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.2 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The F4/80 antibody solution should be stored undiluted at 4C, and protected from prolonged exposure to light. Do not freeze.

Alternative Names

EMR1, Ly71

Regulatory Status

RUO