logo
logo
Antibody for the detection of FLAG(TM)

Cat no: 200-350-383

Antibody for the detection of FLAG(TM)

This agarose-conjugated antibody is optimally suited for immunoprecipitation and purification of FLAG(TM) tagged fusion proteins. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. FLAG(TM) tag can be cleaved by the enterokinase because it contains the Enterokinase Cleavage Site (ECS). Do not use chaotropic reagents such as guanidine HCl; however urea may be used at less than 1M concentration. Do not use any reagent that contains reducing agents such as DTT, DTE, or 2-mercaptoethanol with this product. Do not exceed 5% concentration of TWEEN 20 or TRITON X-100 with this product. Do not exceed 0.1% IGEPAL CA-630 or CHAPS. Do not exceed 0.2% digitonin. Do not exceed 1.0 M sodium chloride. DO NOT USE deoxycholate with this product. DO NOT USE SDS. Keep exposure to glycine HCl to less than 20 minutes.

Prices direct from Rockland Immunochemicals, Inc.

Quick response times

Exclusive Absave savings/discounts

SPECIFICATIONS

Catalog Number

200-350-383

Size

1

Hosts

Mouse

Unit

mL

Format

IgG

Clonality

Monoclonal

Concentration

1 mL agarose-conjugated antibody in 1.5 mL buffer

Storage Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Latest promotions

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...

Buy any polyclonal or monoclonal antibody from our extensive range of pre-made antibodies and for a limited time only receive a $50 discount!(T&C apply:...

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...