Anti-Vero Toxin1 (E. coli) / Shiga Toxin (S. dysenteriae) antibody, rabbit serum

Vero toxin 1 (VT1) is produced by Vero toxin1-producing E.coli (VTEC) and has lethal activity to Vero cells. The primary structure of VT1 is identical or nearly identical to Shiga toxin (Stx) produced by Shigella dysenteriae serotype 1 and also called Slt 1 (Shiga-like toxin 1). VT1 is composed from one A subunit and five B subunits. Some E. coli strains produce both Slt1 and Slt2, and they share sequence identity of 55%, but they are immunologically distict.To express the activity of VT/Stx, interaction with specific receptor Gb3 is indispensable. VT/Stx removes the 4324th adenine of 28S RNA of ribosome, inhibits protein synthesis and causes cell death. After invasion into cell subunit A is cut by furin to give A1 and A2. A1 is an catalytic fragment, and A2 is required for holo-enzyme formation by combining subunit B.