Sup35 protein of S. cerevisiae is the translation termination factor eRF3. The altered conformation of this protein generates the [PSI+] prion phenotype (1). In this state, a dominant cytoplasmically inherited protein aggregates are formed which sequester the normal function of Sup35 thereby nonsense suppressor phenotype is created (2). The molecular chaperon Hsp104 is necessary for the formation and maintenance of the aggregates (3, 4).
Applications
1) Western blotting (1,000~2,000 fold dilution).