anti-rat CD4 pluriBeads

PluriBead is a unique and robust non-magnetic separation technology for cells, proteins, and other targets from nearly all biological fluids. The procedure is simple: You sieve your pluriBeads bound to target cells through a strainer - the pluriBeads with your target cells stay on top, the unwanted cells pass through the strainer. Then gentle detachment of your cells with a buffer is all you do, the target cells are ready to use! PluriBeads are coated with an antibody against a specific target marker. Use the pluriBeads for positive selection or negative depletion of a cell population.Bead Size: MVolume: 2 mlTarget Marker: CD4 antigen is expressed by two-thirds of the peripheral blood T lymphocytes (TH1 and TH2 cells), as well as monocytes, macrophages and granulocytes. The 55 kDa glycoprotein is part of the immunoglobuline superfamily and assists as coreceptor the T cell receptor (TCR). CD4 also serves as coreceptor in MHC class II restricted antigen induced T cell activation and as primary receptor for HIV.Species: RatTarget Cells: Helper T cells, ThymocytesSample Type: PBMC, blood, buffy coat, primary cell solution (also review definitions below)Starting Material: 200 ul blood or 10^5 cells per isolationCapacity: 50 ul for maximum of 2.5 x10^6 target cellsOutput Viability: Greater than 95 percentOutput Purity: Greater than 96 percent_____________Definitions/Applications/Components:S-pluriBeads: 30 um diameter 10^7 maximum target cells per separation 400 ul maximum bead suspension per strainer Sample material – whole blood, tissue, PBMC, cell culture Minimum sample volume is 200 ul Recommended Applications for small number of target cells in a large sample volume and for Circulating Tumor Cells (CTC)M-pluriBeads: 60 um diameter 5 x 10^7 maximum target cells per separation 1000 ul maximum bead suspension per strainer Sample material – whole blood, tissue, PBMC, cell culture, and buffy coat Minimum sample volume is 500 ul Recommended Applications for large number of target cells in sample