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anti-prion antibody(clone 7A1)

Cat no: 65-903

anti-prion antibody(clone 7A1)

Prion protein PrP is a membrane glycosylphosphatidylinositol(GPI) anchored glycoprotein highly expressed in neurons and glial cells, as well as immune and reproductive cells. Mutations, mostly in the octapeptide repeat regions, have been associated with neurodegenerative diseases such as Creutzfeldt Jakob disease, fatal familial insomnia, Gerstmann Straussler disease, Huntington disease like 1, and kuru. The infectious isoform of PrPC, known as PrPSc, is able to induce a conformational change in normal PrPC proteins to convert them into the infectious isoform.Both forms of PrP are initially expressed as a ~250 amino acid protein which is truncated to produce the mature protein of 209 amino acids. Several topological forms exist: one cell surface form which is anchored via a glycolipid modification, and two transmembrane forms. Thus, more than one band is visible on an SDS-PAGE.The mouse monoclonal 7A1 recognizes human prions, not mouse prions. Other species have not been tested. The antibody was produced using recombinant human PrP lacking GPI anchor expressed and purified from rabbit kidney cell line RK13 by N. Kitamoto's laboratory at University of Hyogo.

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SPECIFICATIONS

Catalog Number

65-903

Size

50 ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Clone

7A1

Gene Id

P04156

Clonality

Monoclonal

Dilutions

WB: 0.5 ug/ml

Storage Temp

- 20 degree C

Shipping Temp

4C

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