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Anti-Phospho-Ser<sup>152,156</sup> MARCKS

Anti-Phospho-Ser<sup>152,156</sup> MARCKS

Specific for the ~87k MARCKS protein phosphorylated at Ser152 and Ser156 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda -phosphatase Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

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SPECIFICATIONS

Size

100 ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird, ZF/Fish

Format

Affinity purified, polyclonal antibody

Presku

OAPC00172

Target

Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al., 1990). The phosphorylation of Ser152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 (Palmer et al., 1996) MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994).

Weight

~87kDa

Gene Id

3028

SUPPLIER INFO

Aviva Systems Biology Incorporated

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