Nestin is an intermediate filament protein that is expressed in stem cells and progenitor cells in the mammalian central nervous system (CNS) during development. Nestin is replaced in the adult organism by other intermediate filament proteins, however, it may be re-expressed under certain pathological conditions such as ischemia, inflammation, brain injury, and neoplastic transformation. Nestin has been detected in many kinds of tumors, especially in tumors derived from the CNS, therefore it is considered to be a marker for cancer stem cells in neurogenic tumors. The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes. Recommended applications for this antibody are immunocytochemistry and immunohistochemistry. The antibody does not work well for immunoblotting. However, this antibody is particularly useful for immunostaining of mouse embryonic brain because it is a rat antibody which minimized the immunostaning background when used against mouse tissues. It is also useful for double-staing with mouse and rabbit antibodies.
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