PluriBead is a unique and robust non-magnetic separation technology for cells, proteins, and other targets from nearly all biological fluids.
The procedure is simple: You sieve your pluriBeads bound to target cells through a strainer - the pluriBeads with your target cells stay on top, the unwanted cells pass through the strainer. Then gentle detachment of your cells with a buffer is all you do, the target cells are ready to use!
PluriBeads are coated with an antibody against a specific target marker. Use the pluriBeads for positive selection or negative depletion of a cell population.
Bead Size: S
Volume: 2 ml
Target Marker: CD138 protein, in humans, is encoded by the SDC1 gene. CD138 is a heparin sulfate proteoglycan is a membrane protein that participates in cell proliferation, cell migration, and cell-matrix interactions.
Species: Human
Target Cells:Plasma Cells (plasma B cells, plasmocytes, effector B cells)
Sample Type: PBMC, blood, primary cell solution (also review definitions below)
Starting Material: 200 ul blood or 10^5 cells per isolation
Capacity: 20 ul for maximum of 5 x10^5 target cells
Output Viability: Greater than 95 percent
Output Purity: Greater than 96 percent
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Definitions/Applications/Components:
S-pluriBeads:
30 um diameter
10^7 maximum target cells per separation
400 ul maximum bead suspension per strainer
Sample material – whole blood, tissue, PBMC, cell culture
Minimum sample volume is 200 ul
Recommended Applications for small number of target cells in a large sample volume and for Circulating Tumor Cells (CTC)
M-pluriBeads:
60 um diameter
5 x 10^7 maximum target cells per separation
1000 ul maximum bead suspension per strainer
Sample material – whole blood, tissue, PBMC, cell culture, and buffy coat
Minimum sample volume is 500 ul
Recommended Applications for large number of target cells in sample