The green fluorescent protein (GFP) is composed of 238 amino acids (26.9 kDa), originally isolated from the jellyfish Aequorea victoria that fluoresces green when exposed to blue light (1). In cell and molecular biology, the GFP fused gene is frequently used as a reporter of expression and protein localization (2, 3). The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.
Applications
1. Western blotting (~1ug/ml)
2. Immuno-precipitation
3. Immunocytochemistry
4. Chromatin Immuno-Precipitation (ChIP)
5. ELISA