Background: Cdc37 was initially identified as a cell division cycle control protein of Saccharomyces cerevisiae (1) and was later found to have a much broader role as a molecular chaperone required for folding of protein kinases (2). It forms complex with Hsp90 and a variety of protein kinases and is thought to play a critical role in directing Hsp90 to its target kinases (3). Cdc37 has a molecular weight of 58.4 kD.
Applications
1. Western blotting ( 2,000 fold dilution).
2. Immunoprecipitation.
3. Indirect immuno-staining