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ANAPC2 (Human) cDNA (1ug)

ANAPC2 (Human) cDNA (1ug)

The ORF of human ANAPC2 is amplified by PCR using a high fidelity enzyme and sub-cloned into a Prokaryotic expression vector under the control of the tac promoter. The expression of the gene has been confirmed by Western blot using an anti-ANAPC2 polyclonal antibody.

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SPECIFICATIONS

Size

1ug

Presku

ANA11044

Target

A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation.

Gene Id

29882

Alternative Names

APC2; RP11-350O14.5

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