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Alexa Fluor(R) 700 anti-mouse CD45.1

Alexa Fluor(R) 700 anti-mouse CD45.1

Cat no: 110724

Supplier: BioLegend
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CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Catalogue number: 110724
Reactivities: Mouse
Hosts: Mouse
Applications: Flow Cytometry
Size: 100 microg
Clone: A20
Gene: 19264
Dilutions: This CD45.1 reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ? 0.25 microg per 106 cells in 100 microl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor(R) 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor(R) 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.** Alexa Fluor(R) is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage buffer: The CD45.1 antibody solution should be stored undiluted at 4C, and protected from prolonged exposure to light. Do not freeze.
Concentration: 0.5 mg/ml
Antigen: SJL mouse thymocytes and splenocytes
Isotype: Mouse (A.SW) IgG2a, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: T200, Ly-5.1, LCA
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