Alexa Fluor(R) 700 anti-mouse CD3

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3epsilon, delta, gamma and zeta chains. It forms a TCR complex by associating with TCR alpha/beta or gamma/delta chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

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SPECIFICATIONS

Catalog Number

100216

Size

100 microg

Applications

FC

Hosts

Rat

Reactivities

Mouse

Clone

17A2

Antigen

gammadeltaTCR-positive T-T hybridoma D1

Gene Id

12502

Isotype

Rat IgG2b, kappa

Clonality

Monoclonal

Dilutions

This reagent is developed for immunofluorescent staining for flow cytometric analysis; the suggested use of this reagent is ?1.0 microg per million cells in 100 microl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor(R) 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor(R) 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.** Alexa Fluor(R) 700 is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) 700 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The CD3 antibody solution should be stored undiluted at 4C, and protected from prolonged exposure to light. Do not freeze.

Alternative Names

T cell antigen receptor complex, T3

Regulatory Status

RUO