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Alexa Fluor(R) 647 anti-Phosphotyrosine

Alexa Fluor(R) 647 anti-Phosphotyrosine

Cat no: 309308

Supplier: BioLegend
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Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.
Catalogue number: 309308
Hosts: Mouse
Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence
Size: 100 microg
Clone: PY20
Gene: 5800
Dilutions: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ?0.125 microg per 106 cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application. NOTE: Do not use dilution buffer containing milk as they may interfere with antibody binding to proteins of interest. Dilution and blocking buffers containing 4% bovine serum albumin are recommended for use with this antibody.* Alexa Fluor(R) 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.** Alexa Fluor(R) is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage buffer: The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.
Concentration: 0.5 mg/ml
Antigen: KLH-conjugated phosphotyrosine
Isotype: Mouse IgG2b, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
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