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Alexa Fluor(R) 647 anti-mouse TCR Vbeta8.1, 8.2

Alexa Fluor(R) 647 anti-mouse TCR Vbeta8.1, 8.2

Cat no: 118414

Supplier: BioLegend
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The TCR Vbeta 8 family consists of three members, namely Vbeta8.1, 8.2 and 8.3. KJ16-133.18 reacts with Vbeta8.1, 8.2 only, but not Vbeta8.3. Most mouse strains have about 20% Vbeta 8+ T cells in the periphery.However, C57BR, C57L, SJL, SWR (Tcra haplotype) and RIII strains (Tcrc haplotype) do not express Vbeta8 due to a gene deletion at the Vbeta 8 family loci.Other strains expressing endogenous super-antigen, such as Mls-1a have significant reduced number of Vbeta 8.1 cells in the periphery.Exogenous superantigen, such as staphylococcal enterotoxin B, stimulates lymphocytes bearing Vbeta 8 and selectively eliminate those T cells in vivo.
Catalogue number: 118414
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry
Size: 100 microg
Clone: KJ16-133.18
Gene: 21577
Dilutions: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ? 0.25 microg per 106 cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor(R) 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.** Alexa Fluor(R) is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage buffer: The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.
Concentration: 0.5 mg/ml
Antigen: TCR receptor from T cell hybridoma DO-11.10
Isotype: Rat IgG2a, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: T cell receptor Vb8.1, 8.2 chain, TCR Vb8.1, 8.2
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