Alexa Fluor(R) 647 anti-mouse/rat/human FOXP3 Flow Kit

FOXP3 is a 50 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 delta2) lacking exon 2. The 150D monoclonal antibody reacts with human, mouse and rat FOXP3. The 150D antibody recognizes FOXP3 epitope encoded by exon 2.\nBioLegend's Alexa Fluor(R) 647 anti-mouse/rat/human FOXP3 Flow Kit is designed and formulated specifically for immunofluroscence staining and flow cytometric analysis of mouse/rat/human FOXP3+ Treg cells in a mixed lymphocyte population. This kit is composed of Alexa Fluor(R) 647 conjugated anti-mouse/rat/human FOXP3 antibody and Alexa Fluor(R) 647 conjugated matching isotype control and the critical buffers. It is easy to use for identification of FOXP3+ Treg cells.

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SPECIFICATIONS

Catalog Number

320021

Size

25 tests

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Clone

150D

Gene Id

20371,317382,50943

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 microl per million cells or 5 microl per 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor(R) 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.** Alexa Fluor(R) 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

This kit is guaranteed for three months. Upon receipt, store at 4C and protected from prolonged exposure to light. Do not freeze.

Alternative Names

Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2

Regulatory Status

RUO