Alexa Fluor(R) 647 anti-mouse/human Helios

Helios is a member of the Ikaros family of zinc finger transcription factors. It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members. Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes. It is found at high levels in a subpopulation of regulatory T cells. Helios plays an important role in T cell development and homeostasis. Overexpression of Helios profoundly alters alphabeta T cell differentiation and activation. It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function. Helios is involved in tumor immunity.

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SPECIFICATIONS

Catalog Number

137208

Size

25 tests

Applications

FC, ICC

Reactivities

Hum, Mouse

Clone

22F6

Antigen

Helios peptide (aa 51-107)

Gene Id

22779,22807

Isotype

Armenian Hamster IgG

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by intracellular flow cytometry using our FOXP3 Fix/Perm protocol.For immunofluorescent staining, the suggested use of this reagent is 5 microl per 106 cells in 100 microl volume or 5 microl per 100 microl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\n\n* Alexa Fluor(R) 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.** Alexa Fluor(R) 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.

Regulatory Status

RUO