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Alexa Fluor(R) 647 anti-mouse CD45.1

Alexa Fluor(R) 647 anti-mouse CD45.1

Cat no: 110720

Supplier: BioLegend
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CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Catalogue number: 110720
Reactivities: Mouse
Hosts: Mouse
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry
Size: 100 microg
Clone: A20
Gene: 19264
Dilutions: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ? 0.25 microg per 106 cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for other applications.* Alexa Fluor(R) 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.** Alexa Fluor(R) is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage buffer: The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.
Concentration: 0.5 mg/ml
Antigen: SJL mouse thymocytes and splenocytes
Isotype: Mouse (A.SW) IgG2a, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: T200, Ly-5.1, LCA
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