Alexa Fluor(R) 488 anti-Tubulin-alpha

Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes alpha-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting.

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SPECIFICATIONS

Catalog Number

627905

Size

25 microg

Applications

IF

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Clone

10D8

Antigen

Recombinant full length human alpha-tubulin

Gene Id

84790

Isotype

Mouse IgM, kappa

Clonality

Monoclonal

Dilutions

For immunofluorescent staining applications, a concentration range of 1-4 microg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor(R) 488 has a maximum emission of 519 nm when it is excited at 488 nm.** Alexa Fluor(R) is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.

Alternative Names

alpha-tubulin, Tubulin, alpha-ubiquitous chain

Regulatory Status

RUO