Alexa Fluor(R) 488 anti-mouse FOXP3 Flow Kit

FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 delta2) lacking exon 2. BioLegend's Alexa Fluor(R) 488 anti-mouse FOXP3 Flow Kit is designed and formulated specifically for immunofluorescence staining and flow cytometric analysis of mouse FOXP3+ Treg cells in a mixed lymphocyte population. This kit is composed of Alexa Fluor(R) 488 conjugated anti-mouse FOXP3 antibody and Alexa Fluor(R) 488 conjugated matching isotype control and the critical buffers. It is easy to use for identification of FOXP3+ Treg cells.