Alexa Fluor(R) 488 anti-H2A.X Phospho (Ser139)

H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting and immunofluorescence.

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SPECIFICATIONS

Catalog Number

613405

Size

25 tests

Applications

FC, ICC, IF

Hosts

Mouse

Reactivities

Hum

Clone

2F3

Antigen

Modified peptide

Gene Id

3014

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 microl per million cells. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor(R) 488 has a maximum emission of 519 nm when it is excited at 488 nm.** Alexa Fluor(R) is a registered trademark of Molecular Probes, Inc. Alexa Fluor(R) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.

Alternative Names

H2A.x, H2a/x, Histone 2A, Histone 2A.X

Regulatory Status

RUO