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AFP-ELISA Kit (96 Wells)

Supplier:

Aviva Systems Biology Incorporated

AFP-ELISA Kit (96 Wells)

Principle of the assay: The Aviva AFP ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site on an AFP molecule. An aliquot of sample containing endogenous AFP is incubated in the coated well with enzyme conjugate, which is an anti- AFP antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of AFP in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of AFP in the sample.

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SPECIFICATIONS

Size

96 Wells

Applications

ELISA

Reactivities

Hum

Presku

OKDA00031

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