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Active human PAI-1 functional assay 1 Kit (96 Wells)

Cat no: IHPAIKT-1 Kit (96 Wells)

Active human PAI-1 functional assay 1 Kit (96 Wells)

The sensitive measurement of functionally active PAI-1 is easily performed with this 96 well strip format ELISA kit. Functionally active PAI-1 present in plasma or culture media reacts with urokinase coated onto a micro titer plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. After appropriate washing steps, anti human PAI-1 primary antibody binds to the PAI-1. Excess antibody is washed away, and bound antibody, which is proportional to the original active PAI-1 present in the plasma sample, is then reacted with the secondary antibody conjugated to HRP. TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared using dilutions of purified PAI-1 and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

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SPECIFICATIONS

Catalog Number

IHPAIKT-1 Kit (96 Wells)

Size

EA

Certificate Of Analysis

http://www.innov-research.com/protein-based/452-active-human-pai-1-functional-assay.html

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