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7-AAD Viability Staining Solution

7-AAD Viability Staining Solution

Cat no: 420403

Supplier: BioLegend
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7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).
Catalogue number: 420403
Applications: Flow Cytometry
Size: 200 tests
Dilutions: For dead cell exclusion, resuspend cell pellet in 0.5 mL of Cell Staining Buffer and add 5 microl of 7-AAD per million cells and incubate for 5-10 minutes in the dark before analysis. We recommend utilizing our Fluorescence Spectra Analyzer to determine compatibility of 7AAD with other fluorochromes.
Storage buffer: Protect from light. Store at 4C.
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Viability dye, dead cell exclusion dye
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