Suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers. This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 1.0 M Tris HCl, 1.5 M Sodium Chloride and 0.1% (w/v) Tween-20 at a pH of 7.5.