Hsp70

Detects several members of the heat shock protein 70kDa gene family including Hsp70, Hsc70, p75 and following heat shock, Hsp72 from yeast, Drosophila, fish, mouse, avian, amphibian and human samples.

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SPECIFICATIONS

Catalog Number

SMC-164D

Size

100ug

Applications

ICC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Ch/Bird

Form

Protein G Purified

Clone

3A3

Gene Id

3303

Isotype

IgG1

Accession

NP_005336.3

Dilutions

ICC: 1/500, WB: 1/5000 by ECL; IP: 1-2ug

References

1. Balashova N. et al. (2005) J Biol Chem 280:2186-96. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1): 1-17.\n2. Rothman J. (1989) Cell 59: 591 -601.\n3. DeLuca-Flaherty et al. (1990) Cell 62: 875-887.\n4. Bork P., Sander C. & V

Swiss Prot

P08107

Storage Temp

-20

Concentration

1mg/mL

Research Area

Heat Shock

Shipping Temp

Blue Ice or 4

Storage Buffer

PBS pH7.2, 50% glycerol

Alternative Names

Hsp70 1, Hsp70 2, Hsp70.1, Hsp72, Hsp73, HSPA1, HSPA1A, HSPA1B

Scientific Background

Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic r

Certificate Of Analysis

0.2 ug/mL of SMC-164 was sufficient for detection of Hsp70 in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.