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HDEL

HDEL

Cat no: SMC-175C


Supplier: StressMarq Biosciences Inc.
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Detects ~78kDa.
Catalogue number: SMC-175C
Hosts: Mouse
Applications: Western Blot
Size: 25ug
Clone: 2E7
Accession: N/A
Gene: N/A
Swiss prot: N/A
Dilutions: 1:100-1:1000 (WB), 1:50-1:500 (IF)
Form: Protein G Purified
Storage buffer: PBS pH7.4, 50% glycerol
Concentration: 1mg/mL
Isotype: IgG2B
Shipping temp: Blue Ice or 4
Storage temp: -20
Research area: Heat shock, trafficking
Certificate of analysis: 1 ug/mL of SMC-175 was sufficient for detection of HDEL-containing proteins in 10ug of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Scientific background: HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypept
References: 1. Mayer M.P., and Bukau B. (2005) Cell Mol Life Sci. 62(6): 670-684. 2. Luo S., Mao C., Lee B., and Lee A.S. (2006) Mol Cell Biol. 26(15): 5688-5697. 3. Entrez Gene: HDEL, Gene ID: 10945 4. Napier R.M., et al. (1992) J Cell Sci. 102: 261-271.