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DMPO: FITC

Cat no: SMC-190D

DMPO: FITC

Detects ~90kDa. Recognzies DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.

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SPECIFICATIONS

Catalog Number

SMC-190D

Size

100ug

Hosts

Mouse

Form

Protein G Purified

Clone

N1664A

Isotype

IgG

References

1. Reszka K.J., et al. (2006) Nitric Oxide 15: 133-141. 2. Ramirez D.C., Gomez-Mejiba S.E., and Mason R.P. (2007)\nNat Protoc. 2(3): 512-522.\n3. Khan N., et al. (2003) Free Radic. Biol. Med 34:1473

Storage Temp

-20

Concentration

0.48mg/mL

Research Area

Oxidative Stress

Shipping Temp

Blue Ice or 4

Storage Buffer

TBS pH7.4, 0.05% sodium azide, 50% glycerol

Alternative Names

5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline N oxide

Scientific Background

The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis\nof many disease states (1). The ability to identify these\nspecies is crucial, and spin trapping has accomplished\nthis goal. DMPO (5,5-dimethyl-

Certificate Of Analysis

A 1/1000 dilution of SMC-190 was sufficient to detect the\nDMPO nitrone adducts of metmyoglobin when loaded at\n100ng/lane by colorimetric immunoblot analysis using\nGoat anti-mouse IgG:HRP as the secondary antibody.

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, ICC, IHC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov

More info

Applications

ELISA, ICC, IHC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov

More info

Applications

ELISA, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, GP, Ham, Prc, Rabbit, Sheep

More info

Applications

ELISA, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, GP, Ham, Prc, Rabbit, Sheep

More info

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Ch/Bird, GP, Prc

More info

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