Reagents
Reagents are chemical substances that can be used to detect, measure, examine, block, purify or produce another substance. Reagents are used in biomolecular assays such as ELISA, Immunocytochemistry, Immunohistochemistry, and Western Blot. Some reagents can purify DNA or RNA from tissue, blood, saliva, urine, food or other organic samples. Others can purify and...
...concentrate DNA fragments after PCR reactions, restriction digestion, ligation, and cDNA synthesis.
The reagent chosen for a particular step in any assay must not inadvertently affect other chemicals/steps in that assay. For example, the presence of azide in a reagent might adversely affect the results of an ELISA assay that used horseradish peroxidase-labeled secondary antibodies for detection.
Reagents are used in basic medical, environmental and forensic research; clinical diagnostics and research; and therapeutics, such as neuroscience and regenerative medicine.
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