Recombinant mouse IFN-gamma (ELISA Std.)

Interferon-gamma is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-gamma also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-gamma can upregulate MHC class I and II antigen expression by antigen-presenting cells. The 134 amino acid recombinant mouse IFN-gamma protein has a predicted molecular mass of approximately 15.6 kD.

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SPECIFICATIONS

Catalog Number

575309

Size

4 pack

Applications

ELISA

Gene Id

15978

Dilutions

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 2000 pg/ml to 15 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store unopened vials at 4C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.

Alternative Names

Interferon-gamma, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)

Regulatory Status

RUO