Recombinant Human TGF-alpha (carrier-free)

TGF-alpha was initially identified in the culture medium of several transformed cell lines. TGF-alpha is structurally and functionally related to the epidermal growth factor and belongs to the EGF subfamily that also includes amphiregulin (AR), heparin binding EGF-like growth factor (HB-EGF), betacellulin (BTC), epiregulin (EPR), and epigen (EPG). TGF-alpha is derived from a transmembrane precursor (20-22 kD); pro-TGFalpha undergoes several posttranslational modifications that include N- and O-linked glycosylation and palmitoylation. The pro-TGFalpha includes a glycosylated N-terminal domain, a TGF-alpha region with the EGF-like motif, a linker sequence that joins the ectodomain to the transmembrane domain, and an intracellular portion. TGF-alpha is proteolytically released from the membrane by tumor necrosis factor-alpha converting enzyme (TACE/ADAM-17). TGF-alpha is cleaved at the N- and C-terminus of the EGF motif; the proteolytic cleavage at the C-terminus is required to release the soluble form. The N-terminal cleavage of pro-TGFalpha occurs at the cell surface by TACE-independent activity. TGF-alpha plays a key role regulating follicle development and tumorigenesis. TGF-alpha and its receptor are highly expressed in papillary thyroid carcinoma. Also, TGF-alpha polymorphism has been associated with oral birth defects.

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SPECIFICATIONS

Catalog Number

589904

Size

25 microg

Gene Id

7039

Storage Temp

-20 C or -70 C

Concentration

Shipping Temp

Blue Ice

Storage Buffer

Unopened vial can be stored at 4C for three months, at -20C for six months, or at -70C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 microg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1% BSA or HSA. After dilution, the cytokine can be stored at 4C for one month or from -20C to -70C for up to 3 months. Avoid repeated freeze/thaw cycles.

Alternative Names

TGFA

Regulatory Status

RUO