The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Beta 2-Microglobulin present in samples reacts with the antiBeta 2-Microglobulin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-B2M antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound B2M. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3
Quick Search
Products 
