Rabbit Alpha-2 Macroglobulin ELISA Kit 1 Kit (96 Wells)

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the alpha 2-macroglobulin present in the sample reacts with the anti-A2M antibodies, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound sample proteins by washing, anti-A2M antibodies conjugated with horseradish peroxidase (HRP), are added. This HRP-conjugated antibody forms a complex with the previously bound alpha 2-macroglobulin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3