Purified anti-AGO2 (EIF2C2)

AGO2 is a highly basic protein that contains a PAZ domain and PIWI domain. It is one of the four members of the mammalian Argonaute protein family (AGO1-4). Among the four members, AGO2 is the only member that possesses the slicer endonuclease activity. Argonaute proteins participate in the various steps of microRNA-mediated gene silencing, such as translational repression and mRNA turnover. The important roles of these small, non-coding RNAs on regulating gene expression in higher eukaryotes have been recently identified. The binding of EIF2C2/AGO2 to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA) forms minimal RNA-induced silencing complex (RISC). When RISC binds to a perfectly complementary mRNA, generally it will result in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. In contrast, the binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. Knockout mice studies revealed the essential function of AGO2 in embryonic development in mice.

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SPECIFICATIONS

Catalog Number

654601

Size

25 microg

Applications

WB

Hosts

Mouse

Reactivities

Hum

Clone

9A6SC

Antigen

Partial recombinant human AGO2 protein corresponding to N-terminal region, 200 amino acids.

Gene Id

27161

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 microg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store undiluted at 4C.

Alternative Names

Protein argonaute 2, eIF-2C 2

Regulatory Status

RUO