Purified anti-14-3-3 zeta/delta

The 30 kD 14-3-3 cytoplasmic protein participates in signal transduction by binding to phosphoserine-containing proteins and activating protein kinase C. This protein has also been shown to activate tyrosine/ tryptophan hydroxylases in presence of Ca2+/calmodulin-dependent protein kinase II, regulate insulin sensitivity, inhibit tuberin function, participate in actin cytoskeletal alterations, regulate cell adhesion, and inhibit the pro-apoptotic protein Bad. This protein forms dimers and can be modified by acetylation and phosphorylation. The delta isoform of 14-3-3 is phosphorylated on serine-185; phosphorylation disrupts dimerization. The 14-3-3 protein has been shown to interact with MAPKAPK2, IRS1, PKB/Akt, tuberin, cofilin, LIMK1, ADAM 22, and Bad proteins. The Poly6148 antibody has been shown to be useful for Western blotting of the mouse and human 14-3-3 proten.

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SPECIFICATIONS

Catalog Number

614801

Size

50 microl

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Clone

Poly6148

Antigen

Recombinant (partial), N-terminal

Gene Id

36059

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

-20 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store frozen at -20 C.

Alternative Names

Protein kinase C inhibitor protein-1, Phospholipase A2, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (YWHAZ)

Regulatory Status

RUO