Mouse IL-1a ELISA kit (96 Wells)

Interleukin-1 alpha(IL-1a) is a proinflammatory cytokine produced by a wide variety of cell types, including macrophages, osteoblasts, monocytes and hepatocytes. IL-1 alpha is synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17 kDa. The IL-1a precursor reportedly shows full biological activity in the EL-4 assay. Circulating levels of IL-1a are normally low and only rise after stimulation by agents such as those produced by inflammation, infection or microbial endotoxins. IL-1a possesses a wide variety of biological activities and exerts its effects by binding to specific cell surface receptors.

For the quantitative determination of mouse interleukin 1 alpha (IL-1?) concentrations in cell culture supernates, serum, and plasma.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1? has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1? present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-1? is added to the wells and binds to the combination of capture antibody-IL-1? in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-1? present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-1? standard dilutions and IL-1? sample concentration determined.