Mouse alpha Macroglobulin ELISA Kit (Urine and Cell Culture Samples) 13 Kit (96 Wells)

Mouse alpha Macroglobulin ELISA kit is designed for detection of mouse alpha Macroglobulin in cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures alpha Macroglobulin in 4 hours. A polyclonal antibody specific for alpha Macroglobulin has been pre-coated onto a microplate. Alpha Macroglobulin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for alpha Macroglobulin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.