Human recombinant progelatinase A is expressed in Sf9-insect cells using the baculovirus expression vector system and purified from cell culture supernatant. The full-length recombinant proenzyme consists of 631 amino acids. B-Casein and Gelatin zymography (see Figure A and B with MMP2 in a 12% SDS-PAGE); Recommendation: 5 ul MMP2 (2.5 uU) for B-Casein zymography and 2 ul MMP2 (1 uU) for Gelatin-zymography
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