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human EGF ELISA kit (48 Wells)

human EGF ELISA kit (48 Wells)


Supplier: Aviva Systems Biology Incorporated
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Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF?, amphiregulin (AR), betacellulin (BTC), epiregulin(EPR), heparinbinding EGF-like growth factor (HB-EGF), epigen, and the neuregulins (NRG)1 through-6(1). Members of the EGF family share a structural motif, the EGF-like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues (2). All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The 1207 amino acid (aa) human EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region (3). EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). These receptors undergo a complex pattern of ligand induced homoor heterodimerization to transducer EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

For the quantitative determination of human Epidermal growth factor (EGF) concentrations in cell culture supernates, serum, and plasma
Reactivities: Human
Applications: ELISA
Presku: OKAA00001_48W
Size: 48 Wells