Human C9 ELISA kit is designed for detection of C9 in human plasma, serum, saliva, milk, urine, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures C9 in less than 4 hours. A polyclonal antibody specific for C9has been pre-coated onto a microplate. C9 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for C9, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
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