Infrared-labeled antibodies and controls provided in this kit are sufficient to run approximately 25 immunoblots each measuring 10 cm x 10 cm. The amount of antibody supplied when diluted as recommended in our protocol will yield 1000 ml of working solution. Adjustments in volumes for larger or smaller blots will effect the number of blots detected. Blocking and washing buffers are provided in limited quantities so that the user can immediately begin to process immunoblots. These buffers can be re-ordered in larger quantities for economical use (see ''Replacement Parts'').